Integration of Transcriptome and Proteome Data from Human-Pathogenic Fungi by Using a Data Warehouse

A data warehouse for the integrated storage and visualisation of genome and experimental transcriptome and proteome data of human-pathogenic fungi was established. It provides tools for uploading images and corresponding data from microarray experiments, two-dimensional (2D) gel experiments and mass spectrometry (MS) analyses. All data are cross-linked. A user can find out, on which gels in the database an interesting protein was detected. Additionally, he can see on which microarrays the corresponding mRNA had been spotted and whether these spots show interesting intensity values. So the data warehouse enables an integrated analysis of both transcriptome and proteome data. Some of the uploaded data were transcriptome and proteome time series data of temperature shift experiments obtained from Aspergillus fumigatus. Several proteins were differentially regulated at different times after the temperature shift. For a couple of them also the respective transcripts were found to be differentially expressed. For even more of those proteins the transcripts did not show differential regulation and vice versa. So both kinds of data clearly complement each other and should be analysed together.